Development of a novel method for the simultaneous detection of trimethylamine N-oxide and creatinine in the saliva of patients with chronic kidney disease – Its utility in saliva as an alternative to blood

肾功能 肌酐 唾液 化学 肾脏疾病 氧化三甲胺 泌尿科 胱抑素C 分析物 色谱法 尿 内科学 内分泌学 三甲胺 医学 生物化学
作者
Natalia Korytowska,Sylwia Michorowska,Aleksandra Wyczałkowska-Tomasik,Leszek Pączek,Joanna Giebułtowicz
出处
期刊:Journal of Pharmaceutical and Biomedical Analysis [Elsevier]
卷期号:234: 115519-115519
标识
DOI:10.1016/j.jpba.2023.115519
摘要

Chronic kidney disease (CKD) is associated with increased levels of creatinine and other uremic toxins (UTs), which impaired kidneys cannot filtrate. Typically, CKD is diagnosed by calculating the estimated glomerular filtration rate using serum creatinine or cystatin C levels. In pursuit of more sensitive and reliable biomarkers of kidney dysfunction, scientific attention has turned towards other UTs, such as trimethylamine N-oxide (TMAO), successfully quantified in standard matrices, blood and urine. However, less invasive monitoring of kidney function can be performed using an alternative diagnostic biofluid, saliva, which has been shown to contain clinically relevant concentrations of renal function markers. Accurate quantitative estimation of serum biomarkers using saliva measurements can only be achieved provided that there is a tight saliva-serum correlation for the analyte of interest. Therefore, we aimed to verify the correlation between saliva and serum levels of TMAO in CKD patients using newly developed and validated quantitative liquid chromatography coupled to mass spectrometry (LC-MS) method for simultaneous detection of TMAO, and creatinine – the conventional marker of renal impairment. Secondly, we applied this method to quantify TMAO and creatinine levels in the resting saliva of CKD patients collected with a standardised method involving swab-based collectors. A good linear correlation was obtained between the concentration of creatinine in serum and resting saliva of CKD patients (r = 0.72, p = 0.029) and even better in the case of TMAO (r = 0.81, p = 0.008). The analysed validation criteria were fulfilled. No significant influence of the type of swab in the Salivette® device on creatinine and TMAO concentrations in saliva was detected. Our study indicates that saliva can be successfully used in the non-invasive monitoring of renal failure in CKD by measuring salivary TMAO concentrations.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
华仔应助浮生采纳,获得10
1秒前
路内里发布了新的文献求助10
2秒前
2秒前
斯文败类应助himan采纳,获得10
2秒前
3秒前
笨笨甜瓜完成签到,获得积分20
5秒前
打打应助必胜客采纳,获得10
6秒前
7秒前
Phosphene应助去看海嘛采纳,获得10
7秒前
8秒前
科研通AI2S应助Yuan采纳,获得10
8秒前
端庄的白开水完成签到,获得积分10
8秒前
8秒前
善良宛筠发布了新的文献求助10
9秒前
himan完成签到,获得积分10
10秒前
11秒前
11秒前
星星发布了新的文献求助10
12秒前
神内打工人完成签到 ,获得积分10
13秒前
ref:rain完成签到,获得积分10
13秒前
科研通AI2S应助笨笨甜瓜采纳,获得10
13秒前
14秒前
HH完成签到,获得积分10
14秒前
FashionBoy应助不要引力采纳,获得10
15秒前
guyanlong发布了新的文献求助10
15秒前
韦以亦发布了新的文献求助10
16秒前
himan发布了新的文献求助10
16秒前
不许内耗完成签到,获得积分20
16秒前
17秒前
18秒前
那些年发布了新的文献求助10
18秒前
18秒前
科目三应助Yuan采纳,获得10
18秒前
星星完成签到,获得积分10
18秒前
19秒前
yufanhui应助不许内耗采纳,获得10
20秒前
虬江学者发布了新的文献求助10
22秒前
桐桐应助treelet007采纳,获得10
23秒前
23秒前
赘婿应助必胜客采纳,获得10
24秒前
高分求助中
Sustainability in ’Tides Chemistry 2000
Studien zur Ideengeschichte der Gesetzgebung 1000
The ACS Guide to Scholarly Communication 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Handbook of the Mammals of the World – Volume 3: Primates 805
Ethnicities: Media, Health, and Coping 800
Gerard de Lairesse : an artist between stage and studio 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3072205
求助须知:如何正确求助?哪些是违规求助? 2726027
关于积分的说明 7492250
捐赠科研通 2373536
什么是DOI,文献DOI怎么找? 1258633
科研通“疑难数据库(出版商)”最低求助积分说明 610333
版权声明 596952