转录组
结直肠癌
转移
癌症研究
生物
CD8型
细胞
癌症
免疫系统
免疫学
基因表达
基因
遗传学
作者
Fei Wang,Jie Long,Liang Li,Zixin Wu,Tian-Tian Da,Xiaoqing Wang,Chuan Huang,Yihua Jiang,Xueqing Yao,Haiqing Ma,Zhe‐Xiong Lian,Z Zhao,Jie Cao
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2023-06-16
卷期号:9 (24)
被引量:50
标识
DOI:10.1126/sciadv.adf5464
摘要
In this study, we comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing. We generated 41,892 CD45 − nonimmune cells and 196,473 CD45 + immune cells from 27 samples of six CRC patients, and found that CD8_CXCL13 and CD4_CXCL13 subsets increased significantly in liver metastatic samples that exhibited high proliferation ability and tumor-activating characterization, contributing to better prognosis of patients. Distinct fibroblast profiles were observed in primary and liver metastatic tumors. F3 + fibroblasts enriched in primary tumors contributed to worse overall survival by expressing protumor factors. However, MCAM + fibroblasts enriched in liver metastatic tumors might promote generation of CD8_CXCL13 cells through Notch signaling. In summary, we extensively analyzed the transcriptional differences of cell atlas between primary and liver metastatic tumors of CRC by single-cell and spatial transcriptome RNA sequencing, providing different dimensions of the development of liver metastasis in CRC.
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