Correlation of the surface expression of thymic stromal lymphopoietin receptor with the presence of CRLF2 gene rearrangements in children with B‐lineage acute lymphoblastic leukemia

流式细胞术 胸腺基质淋巴细胞生成素 免疫分型 医学 间质细胞 淋巴细胞白血病 队列 基因 相关性 癌症研究 分子生物学 受体 免疫学 白血病 内科学 生物 遗传学 数学 几何学
作者
I. A. Demina,Elena Zerkalenkova,Olga Soldatkina,Anna Kazakova,Alexandra Semchenkova,Maria Goncharova,Galina Novichkova,Michael Maschan,Alexander Karachunskiy,Yulia Olshanskaya,А. М. Попов
出处
期刊:International Journal of Laboratory Hematology [Wiley]
卷期号:45 (3): 337-343 被引量:1
标识
DOI:10.1111/ijlh.14028
摘要

In this study, we aimed to compare the immunophenotype of tumor cells in children with B-cell precursor acute lymphoblastic leukemia (BCP-ALL) harboring rearrangements of the CRLF2 gene with that in children without such aberrations with a specific focus on the surface expression of the related protein thymic stromal lymphopoietin receptor (TSLPR).We examined bone marrow samples from 46 patients with primary BCP-ALL who had CRLF2 rearrangements detected by FISH (CRLF2(+) cohort). A total of 140 consecutive patients with intact CRLF2 were included in a control CRLF2(-) cohort. TSLPR expression was studied by flow cytometry.The majority of CRLF2(+) patients were conventionally positive (≥20% positive cells) for TSLPR (33 of 46, 71.7%). Among the remaining children in this group, two were completely TSLPR-negative, seven had less than 10% TSLPR-positive cells, and four had between 10% and 20% TSLPR-positive cells. By contrast, the majority of CRLF2(-) patients had no TSLPR-positive cells (119 of 140, 85.0%), while in 15 cases (10.7%), the percentage of TSLPR-positive cells was below 10%, and in six cases (4.3%), it was between 10% and 20%. Receiver operator characteristic analysis revealed a threshold of only 1.6% TSLPR-positive cells for the effective prediction of the presence of CRLF2 rearrangement. Moreover, this threshold retained its predictive value when only children with low TSLPR positivity were studied.When surface TSLPR is detected at the diagnosis of BCP-ALL, close attention should be given to the search for chromosomal aberrations involving CRLF2 at any level of expression.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Lucas应助pomfret采纳,获得10
刚刚
1秒前
1秒前
科目三应助娇气的天亦采纳,获得10
2秒前
JiangHan发布了新的文献求助10
2秒前
4秒前
GGBOND发布了新的文献求助10
4秒前
5秒前
黄振全发布了新的文献求助10
5秒前
6秒前
Lu发布了新的文献求助10
7秒前
苯氮小羊发布了新的文献求助10
7秒前
hugdoggy发布了新的文献求助10
9秒前
9秒前
英姑应助shawn采纳,获得10
12秒前
完美世界应助22采纳,获得10
12秒前
Hululu完成签到,获得积分10
12秒前
13秒前
精美礼物发布了新的文献求助30
14秒前
anna发布了新的文献求助10
14秒前
GGBOND发布了新的文献求助10
19秒前
pomfret完成签到,获得积分10
19秒前
从容甜瓜完成签到 ,获得积分10
21秒前
量子星尘发布了新的文献求助10
21秒前
22秒前
23秒前
桐桐应助Lu采纳,获得10
24秒前
YWang发布了新的文献求助10
26秒前
han应助归华采纳,获得10
27秒前
27秒前
SYLH应助zly采纳,获得30
30秒前
完美世界应助娇气的天亦采纳,获得10
32秒前
34秒前
37秒前
科目三应助彭栋采纳,获得10
39秒前
方文浩发布了新的文献求助10
39秒前
ding应助YWang采纳,获得10
42秒前
42秒前
林宝雯关注了科研通微信公众号
47秒前
50秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989115
求助须知:如何正确求助?哪些是违规求助? 3531367
关于积分的说明 11253688
捐赠科研通 3269986
什么是DOI,文献DOI怎么找? 1804868
邀请新用户注册赠送积分活动 882078
科研通“疑难数据库(出版商)”最低求助积分说明 809105