Identification of chemical constituents and absorbed components in rat serum of Ipomoea muricata (L.) Jacq. by UPLC-Q-exactive-MS

Ipomoea muricata (L.) Jacq. has anti-inflammatory, analgesic, haemostatic and antioxidant effects, but its pharmacological components are still unclear. This study comprehensively identified chemical constituents and absorbed components of I. muricata by UPLC-Q-Exactive-MS. The column temperature was 35 °C, and the injection volume was 2 μl. The mobile phase using a 0.1% formic acid as phase A and 0.1% formic acid of solution in methanol as phase B was adopted in positive ion mode. And the mobile phase using a 10 mM formic ammonia solution as phase A and 10 mM formic ammonia of solution in 95% methanol as phase B was adopted in negative ion mode. In this study, we identified 25 compounds from the alcoholic extract of I. muricata, including 11 organic acids, 5 coumarins, 2 flavonoids, 7 alkaloids and nitrogen-containing compounds. Nine absorbed components were identified from the serum of rats after administration, including adenosine, p-hydroxybenzoic acid, chlorogenic acid, dextroquinic acid, shikimic acid, caffeic acid, p-hydroxycinnamic acid, chrysin, and umbelliferone. UPLC-Q-Exactive-MS technique can quickly identify the chemical constituents and absorbed components in rat serum of I. muricata, and provide scientific basis for clarifying the pharmacodynamic material basis and optimising the quality control index of I. muricata.