Metabolic regulation of Shewanella oneidensis for microbial electrosynthesis: From extracellular to intracellular

舍瓦内拉 格式化 甲酸脱氢酶 细胞内 生物化学 细胞外 希瓦氏菌属 突变体 电合成 脱氢酶 生物 化学 基因 细菌 电化学 遗传学 电极 物理化学 催化作用
作者
Yixin Li,Qingliu Luo,Jiaying Su,Guowen Dong,Mingfeng Cao,Yuanpeng Wang
出处
期刊:Metabolic Engineering [Elsevier BV]
卷期号:80: 1-11 被引量:12
标识
DOI:10.1016/j.ymben.2023.08.004
摘要

Shewanella oneidensis MR-1 (S. oneidensis MR-1) has been shown to benefit from microbial electrosynthesis (MES) due to its exceptional electron transfer efficiency. In this study, genes involved in both extracellular electron uptake (EEU) and intracellular CO2 conversion processes were examined and regulated to enhance MES performance. The key genes identified for MES in the EEU process were mtrB, mtrC, mtrD, mtrE, omcA and cctA. Overexpression of these genes resulted in 1.5-2.1 times higher formate productivity than that of the wild-type strains (0.63 mmol/(L·μg protein)), as 0.94-1.61 mmol/(L·μg protein). In the intracellular CO2 conversion process, overexpression of the nadE, nadD, nadR, nadV, pncC and petC genes increased formate productivity 1.3-fold-3.4-fold. Moreover, overexpression of the formate dehydrogenase genes fdhA1, fdhB1 and fdhX1 in modified strains led to a 2.3-fold-3.1-fold increase in formate productivity compared to wild-type strains. The co-overexpression of cctA, fdhA1 and nadV in the mutant strain resulted in 5.59 times (3.50 mmol/(L·μg protein)) higher formate productivity than that of the wild-type strains. These findings revealed that electrons of MES derived from the electrode were utilized in the energy module for synthesizing ATP and NADH, followed by the synthesis of formate in formate dehydrogenase by the combinatorial effects of ATP, NADH, electrons and CO2. The results provide new insights into the mechanism of MES in S. oneidensis MR-1 and pave the way for genetic improvements that could facilitate the further application of MES.
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