适体
循环肿瘤细胞
胶体金
检出限
生物传感器
电化学
电极
DNA
线性范围
离心
纳米颗粒
癌细胞
化学
纳米技术
材料科学
生物物理学
分子生物学
癌症
色谱法
生物化学
生物
转移
物理化学
遗传学
作者
Nong Yu,Guifeng Ma,Yuyao Chen,Qi Xiao,Ying Gong,Shuangshuang Li,Haibin Gu,Honglan You,Peng Miao
标识
DOI:10.1016/j.colsurfb.2023.113482
摘要
The concentration of circulating tumor cells (CTCs) in peripheral blood is strongly correlated with the progress of certain metastatic cancers. In this study, we have developed a novel and facile electrochemical biosensor for the detection of CTCs based on the use of manganese dioxide nanosheets (MnO2 NSs) and gold nanoparticles (AuNPs). Aptamer sequence of target cell is modified on the surface of AuNPs for specifical recognition. With low-speed centrifugation, numerous AuNPs@DNA can be removed from the supernatant. On the other hand, MnO2 NSs are modified on the electrode surface to capture unreacted AuNPs@DNA. The declined electrochemical signal intensity can be used to reflect the level of CTCs. This biosensor achieves a wide linear range from 10 to 104 cells mL-1 and a limit of detection as low as 3 cells mL-1. Due to the specific aptamer as the recognition element, interfering cells can be successfully distinguished and this method performs satisfactorily in clinical samples. Therefore, it has great potential to be used as a powerful tool benefiting rare cells analysis and the investigation of dynamics of cellular interactions.
科研通智能强力驱动
Strongly Powered by AbleSci AI