SlMYB41 positively regulates tomato thermotolerance by activating the expression of SlHSP90.3

High-temperature stress has become a major abiotic factor that dramatically limits plant growth and crop yield. Plants have evolved complex mechanisms to cope with high-temperature stress, but the factors that regulate plant thermotolerance remain to be discovered. Here, a high temperature-induced MYB transcription factor SlMYB41 was cloned from tomato (Solanum lycopersicum). Two individual SlMYB41-RNA interference (RNAi) lines (MR) and one CRISPR/Cas9 mediated myb41 mutant (MC) were obtained to investigate the function of SlMYB41 in tomato thermotolerance. Under high-temperature stress, we found that the MR and MC lines showed more wilting than the wild type (WT), with more ion leakage, more MDA accumulation, lower contents of osmotic adjustment substances, and more accumulation of reactive oxygen species (ROS) which was resulted from lower antioxidative enzyme activities. In addition, the photosynthetic capacity and complex of MR and MC lines were damaged more seriously than WT plants under high-temperature stress, mainly manifested in lower photosynthetic rate and Fv/Fm. Moreover, heat stress-related genes, such as SlHSP17.6, SlHSP17.7, and SlHSP90.3 were downregulated in MR and MC lines. Importantly, Y1H and LUC analysis indicated that SlMYB41 can directly activate the transcription of SlHSP90.3. Together, our study suggest that SlMYB41 positively regulates tomato thermotolerance by activating the expression of SlHSP90.3.