花青素
原花青素
草莓
MYB公司
类黄酮生物合成
类黄酮
植物
生物
化学
生物化学
多酚
转录因子
基因表达
基因
转录组
抗氧化剂
作者
Lianxi Zhang,Yiping Wang,Maolan Yue,Leiyu Jiang,Nating Zhang,Ya Luo,Qing Chen,Yong Zhang,Yan Wang,Mengyao Li,Yong Zhang,Yuanxiu Lin,Haoru Tang
标识
DOI:10.3390/ijms241512185
摘要
MYB and BBX transcription factors play important roles in flavonoid biosynthesis. Here, we obtained transgenic woodland strawberry with stable overexpression of FaMYB5, demonstrating that FaMYB5 can increase anthocyanin and proanthocyanidin content in roots, stems and leaves of woodland strawberry. In addition, bimolecular fluorescence complementation assays and yeast two-hybridization demonstrated that the N-terminal (1-99aa) of FaBBX24 interacts with FaMYB5. Transient co-expression of FaBBX24 and FaMYB5 in cultivated strawberry ‘Xiaobai’ showed that co-expression strongly promoted the expression of F3′H, 4CL-2, TT12, AHA10 and ANR and then increased the content of anthocyanin and proanthocyanidin in strawberry fruits. We also determined that FaBBX24 is also a positive regulator of anthocyanin and proanthocyanidin biosynthesis in strawberry. The results reveal a novel mechanism by which the FaMYB5–FaBBX24 module collaboratively regulates anthocyanin and proanthocyanidin in strawberry fruit.
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