纳米材料
枯草芽孢杆菌
辅因子
化学
酶
木糖
木糖醇
纳米技术
生物化学
材料科学
细菌
发酵
生物
遗传学
作者
NULL AUTHOR_ID,Ranran Wu,NULL AUTHOR_ID,F.L. Li,Yuanming Wang,NULL AUTHOR_ID,NULL AUTHOR_ID,NULL AUTHOR_ID
标识
DOI:10.1016/j.bioelechem.2024.108777
摘要
To take advantage of the high specificity of enzymatic catalysis along with the high efficiency of electrochemical cofactor regeneration, a bacterial surface displayed enzyme-nanomaterial hybrid bioelectrocatalytic system is herein developed. A cofactor-dependent xylose reductase, capable of reducing xylose to xylitol, is displayed on the surface of Bacillus subtilis, followed by the attachment of copper nanomaterials via the binding of His-tagged enzyme with the nickel ion. This hybrid system can regenerate NADPH with a highest efficiency of 71.6 % in 4 h without the usage of extra electron mediators, and 2.35 mM of xylitol can be synthesized after a series of optimization processes. This work opens up new possibilities for the construction and application of bioelectrocatalytic systems with enzyme-nanomaterial hybrids.
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