生物
回肠
淋巴细胞
背景(考古学)
免疫学
先天性淋巴细胞
细胞
电池类型
细胞生物学
先天免疫系统
免疫系统
遗传学
内分泌学
古生物学
作者
Jayne E Wiarda,Julian Trachsel,Sathesh K. Sivasankaran,Christopher K. Tuggle,Crystal L. Loving
出处
期刊:Life science alliance
[Life Science Alliance]
日期:2022-08-22
卷期号:5 (10): e202201442-e202201442
被引量:21
标识
DOI:10.26508/lsa.202201442
摘要
Lymphocytes can heavily influence intestinal health, but resolving intestinal lymphocyte function is challenging as the intestine contains a vastly heterogeneous mixture of cells. Pigs are an advantageous biomedical model, but deeper understanding of intestinal lymphocytes is warranted to improve model utility. Twenty-six cell types were identified in the porcine ileum by single-cell RNA sequencing and further compared with cells in human and murine ileum. Though general consensus of cell subsets across species was revealed, some porcine-specific lymphocyte subsets were identified. Differential tissue dissection and in situ analyses conferred spatial context, revealing similar locations of lymphocyte subsets in Peyer’s patches and epithelium in pig-to-human comparisons. Like humans, activated and effector lymphocytes were abundant in the ileum but not periphery of pigs, suggesting tissue-specific and/or activation-associated gene expression. Gene signatures for peripheral and ileal innate lymphoid cells newly discovered in pigs were defined and highlighted similarities to human innate lymphoid cells. Overall, we reveal novel lymphocyte subsets in pigs and highlight utility of pigs for intestinal research applications.
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