亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Mitochondrial H2O2 metabolism as central event of heart complex I syndrome in early diabetes

内科学 线粒体 内分泌学 链脲佐菌素 糖尿病 氧化应激 胰岛素 线粒体内膜 过氧化氢酶 过氧化脂质 脂质过氧化 线粒体ROS 化学 生物 医学 生物化学
作者
Ivana A. Rukavina-Mikusic,Micaela Rey,Juan Santiago Adán Areán,Virginia Vanasco,Silvia Álvarez,Laura B. Valdez
出处
期刊:Free Radical Biology and Medicine [Elsevier BV]
卷期号:201: 66-75 被引量:2
标识
DOI:10.1016/j.freeradbiomed.2023.03.011
摘要

Hydrogen peroxide is the main metabolite effective in redox regulation and it is considered an insulinomimetic agent, with insulin signalling being essential for normal mitochondrial function in cardiomyocytes. Therefore, the aim of this work was to deeply analyse the heart mitochondrial H2O2 metabolism, in the early stage of type 1 diabetes. Diabetes was induced by Streptozotocin (STZ, single dose, 60 mg × kg-1, ip.) in male Wistar rats and the animals were sacrificed 10 days after injection. Mitochondrial membrane potential and ATP production, using malate-glutamate as substrates, in the heart of diabetic animals were like the ones observed in control group. Mn-SOD activity was lower (15%) in the heart of diabetic rats even though its expression was increased (29%). The increment in heart mitochondrial H2O2 production (117%) in diabetic animals was accompanied by an enhancement in the activities and expressions of glutathione peroxidase (26% and 42%) and of catalase (200% and 133%), with no changes in the peroxiredoxin activity, leading to [H2O2]ss ∼40 nM. Heart mitochondrial lipid peroxidation and protein nitration were higher in STZ-injected animals (45% and 42%) than in control group. The mitochondrial membrane potential and ATP production preservation suggest the absence of irreversible damage at this early stage of diabetes 1. The increase in mitochondrial [H2O2]ss above the physiological range, but still below supraphysiological concentration (∼100 nM) seems to be part of the adaptive response triggered in cardiomyocytes due to the absence of insulin. The signs of mitochondrial dysfunction observed in this very early stage of diabetes are consistent with the mitochondrial entity called ″complex I syndrome″.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
25秒前
陈苗发布了新的文献求助10
30秒前
在水一方应助scholar丨崔采纳,获得10
47秒前
不能随便完成签到,获得积分10
52秒前
1分钟前
碧蓝香芦完成签到 ,获得积分10
1分钟前
scholar丨崔发布了新的文献求助10
1分钟前
1分钟前
1分钟前
可爱的函函应助陈苗采纳,获得10
1分钟前
北北完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
Nowind发布了新的文献求助10
1分钟前
三叔发布了新的文献求助10
1分钟前
三叔完成签到,获得积分0
1分钟前
怕孤单的绿柏完成签到,获得积分10
1分钟前
Augustines完成签到,获得积分10
2分钟前
2分钟前
lijunliang完成签到 ,获得积分10
2分钟前
Lucas应助欣喜的以丹采纳,获得10
2分钟前
爱吃煎饼果子的芋圆完成签到 ,获得积分10
2分钟前
2分钟前
小神仙完成签到 ,获得积分10
2分钟前
尊敬夜南发布了新的文献求助10
2分钟前
sss发布了新的文献求助10
2分钟前
123发布了新的文献求助10
2分钟前
科研通AI5应助sss采纳,获得10
3分钟前
3分钟前
章水云发布了新的文献求助10
3分钟前
psyche完成签到,获得积分10
3分钟前
fighting完成签到,获得积分10
3分钟前
小马甲应助sljzhangbiao11采纳,获得10
3分钟前
大模型应助最溜皮大爷采纳,获得10
4分钟前
所所应助喂喂采纳,获得10
4分钟前
5High_0完成签到 ,获得积分10
4分钟前
4分钟前
陈苗发布了新的文献求助10
4分钟前
uuuuu完成签到,获得积分10
4分钟前
优美的谷完成签到,获得积分10
4分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990022
求助须知:如何正确求助?哪些是违规求助? 3532092
关于积分的说明 11256327
捐赠科研通 3270943
什么是DOI,文献DOI怎么找? 1805140
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228