结晶紫
染色
程序性细胞死亡
活力测定
细胞
化学
人口
细胞培养
分子生物学
细胞生物学
细胞存活
生物
细胞凋亡
生物化学
微生物学
医学
遗传学
环境卫生
作者
Maria Feoktistova,Peter Geserick,Martin Leverkus
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2016-04-01
卷期号:2016 (4): pdb.prot087379-pdb.prot087379
被引量:637
标识
DOI:10.1101/pdb.prot087379
摘要
Adherent cells detach from cell culture plates during cell death. This characteristic can be used for the indirect quantification of cell death and to determine differences in proliferation upon stimulation with death-inducing agents. One simple method to detect maintained adherence of cells is the staining of attached cells with crystal violet dye, which binds to proteins and DNA. Cells that undergo cell death lose their adherence and are subsequently lost from the population of cells, reducing the amount of crystal violet staining in a culture. This protocol describes a quick and reliable screening method that is suitable for the examination of the impact of chemotherapeutics or other compounds on cell survival and growth inhibition. However, characterization of the cause of reduced crystal violet staining requires additional methods detailed elsewhere.
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