连接酶连锁反应
基因分型
沙眼衣原体
核酸
聚合酶链反应
生物
计算生物学
病毒学
基因型
遗传学
多重聚合酶链反应
基因
作者
William H. Benjamin,Kim Smith,Ken B. Waites
出处
期刊:Methods in molecular biology
日期:2003-01-01
卷期号:: 135-150
标识
DOI:10.1385/1-59259-384-4:135
摘要
AbstractThe ligase chain reaction (LCR) is one of many techniques developed in recent years to detect specific nucleic acid sequences by amplification of nucleic acid targets. The LCR has been used for genotyping studies to detect tumors and identify the presence of specific genetic disorders such as sickle cell disease caused by known nucleotide changes that occur as a result of point mutations and has now become widely used in infectious disease detection, both in the diagnostic and research settings, primarily focusing on infections caused by microbes that have proven difficult to detect by traditional culture techniques. The LCR is now recognized as the method of choice for detection of urogenital infections due to Chlamydia trachomatis because of its greater sensitivity as compared to traditional cell culture or nonamplifed DNA probes or antigen-detection assays. Other uses of the LCR have also been reported (1–8). When used for detection of infectious diseases, amplification tests such as the LCR have the additional advantages in that they do not require viable organisms in a specimen, a single specimen can be used to detect multiple different pathogens, provided suitable primers are available, and easily obtained specimens such as urine can be used for diagnostic purposes, making screening of large numbers of persons practical, as well as facilitating research to better understand the epidemiology of specific diseases.KeywordsChlamydia TrachomatisUrine SpecimenAbbott DiagnosticsLigase Chain ReactionLigase Detection ReactionThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
科研通智能强力驱动
Strongly Powered by AbleSci AI