Identification of endophytic fungi SIPI3.0550 and its metabolites

Objective: To identify the endophytic fungi SIPI3.0550 and to study its metabolites.Methods: Solid-state fermentation was used to culture the endophytic fungi.The activity of the fermentation extracts against Gram-positive bacteria(G+),Gram-negative bacteria(G-) and fungi was determined.The methods of solvent extraction,macroporous resin column chromatography and crystallization were used to separate and purify the liposoluble components of SIPI3.0550.Structures of the purified compounds were elucidated by physico-chemical properties and spectral analysis.Results: Two compounds were separated and identified,i.e.,monomethylsulochrin(1) and trypacidin(2).The compound 2 showed the activity against G+ and Methicillin-resistant Staphylococcus aureus(MRSA).Conclusion: Bioactive metabolites with a higher anti-MRSA activity are obtained from endophytic fungi SIPI3.0550.