Three New C21 Steroidal Glycosides from Roots of Cynanchum otophyllum

Abstract Objective To investigate the chemical structures of glycosides in the roots of Cynanchum otophyllum (Asclepiadaceae) and to find new glycosides. Methods The total glycosides in the roots of C. olophyllum were separated by silica gel column chromatography. The structures of the resulting compounds were determined by NMR and FAB-MS spectra. Results Three C21 steroidal glycosides were separated. Their structures were determined as caudatin 3- O -(4- O -methyl-β- D -cymaropyranosyl)-(1→4)-α- D -oleandropyranosyl-(1→4)-β- D -glucopyranosyl-(1→4)-α- L -rhamnopyranoside ( 1 ), caudatin 3- O -β- D -digitoxopyranosyl-(1→4)-α- D -oleandropyranosyl-(1→4)-β- D diginopyranosyl-(1→4)-β- D -glucopyranoside ( 2 ), and caudatin 3- O -β- D -diginopyranosyl-(1→4)-α- D -oleandropyranosyl-(1→4)-α- D -oleandropyranosyl-(1→4)-β- D -glucopyranoside ( 3 ), respectively. Conclusion Glycosides 1–3 are new compounds.