小发夹RNA
体内
生物
微气泡
基因传递
药理学
遗传增强
癌症研究
超声波
分子生物学
医学
细胞凋亡
生物化学
基因敲除
基因
放射科
生物技术
作者
D H Park,Byung Mun Jung,Yun-Hee Lee,Jae-Yeon Jang,M K Kim,J K Lee,H Park,Jin Sung Seo,C W Kim
出处
期刊:Gene Therapy
[Springer Nature]
日期:2015-01-15
卷期号:22 (4): 325-332
被引量:20
摘要
Gene therapy using RNA interference can be directed against tumors through various strategies, but has been hindered owing to the inefficiency of non-viral delivery. To evaluate the antitumor effects of adenine nucleotide translocase-2 (ANT2) short hairpin RNA (shRNA) by intraperitoneal injection using the polyethylenimine (PEI) and an ultrasound gene delivery method, human breast carcinoma MDA-MB-231 cells were injected subcutaneously into NOG (NOD/Shi-scid/IL-2Rγ(null)) mice. The results showed greater tumor regression (*P<0.05) as well as an increased survival rate in the group receiving ANT2 shRNA+two types of enhancer relative to the groups receiving ANT2 shRNA without enhancer. These findings demonstrate that the introduction of PEI and ultrasound with SonoVue exerted enhanced antitumor effects in vivo. Although the combination of jet-PEI and ultrasound provided the best results with respect to tumor regression, the antitumor effects from the individual enhancers were approximately equivalent. In addition, we confirmed that there was no toxicity on aspartate aminotransferase and alanine aminotransferase levels in the liver and albumin, blood urea nitrogen or creatine kinase levels in the kidney following the various gene delivery methods.
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