Nickel oxide nanoparticles induce inflammation and genotoxic effect in lung epithelial cells

活力测定 A549电池 细胞凋亡 内吞作用 细胞内 细胞生物学 化学 MAPK/ERK通路 细胞毒性T细胞 DNA损伤 炎症 细胞周期 激酶 细胞 分子生物学 生物 生物化学 免疫学 体外 DNA
作者
Laura Capasso,Marina Camatini,Maurizio Gualtieri
出处
期刊:Toxicology Letters [Elsevier BV]
卷期号:226 (1): 28-34 被引量:155
标识
DOI:10.1016/j.toxlet.2014.01.040
摘要

Nickel oxide nanoparticles (NiONPs) toxicity has been evaluated in the human pulmonary epithelial cell lines: BEAS-2B and A549. The nanoparticles, used at the doses of 20, 40, 60, 80, 100 μg/ml, induced a significant reduction of cell viability and an increase of apoptotic and necrotic cells at 24h. A significant release of interleukin-6 and -8 was assessed after 24h of treatment, even intracellular ROS increased already at 45 min after exposure. The results obtained evidenced that the cytokines release was dependent on mitogen activated protein kinases (MAPK) cascade through the induction of NF-kB pathway. NiONPs induced cell cycle alteration in both the cell lines even in different phases and these modifications may be induced by the NPs genotoxic effect, suggested by the nuclear translocation of phospho-ATM and phospho-ATR. Our results confirm the cytotoxic and pro-inflammatory potential of NiONPs. Moreover their ability in inducing DNA damage responses has been demonstrated. Such effects were present in A549 cells which internalize the NPs and BEAS-2B cells in which endocytosis has not been observed.
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