免疫分析
放射免疫扩散
色谱法
化学
荧光
抗体
免疫学
生物
量子力学
物理
作者
Michael W. Bürgett,Sandra Fairfield,James F. Monthony
标识
DOI:10.1016/0022-1759(77)90199-5
摘要
A non-competitive method for the determination of the C4 component of human complement in serum is described. This procedure involves use of a specific antibody covalently attached to derivatized polyacrylamide beads and a fluorescently labeled specific antibody. Reproducible results were achieved for C4 in serum in the range of 10 mg/dl to 170 mg/dl within 2h. C4 levels as low as 150 ng/ml can also be measured. Fluorescent immunoassay and radial immunodiffusion were used to determine C4 levels in healthy adults. Good agreement was found between the two methods.
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