生物
生物医学
基因
计算生物学
生物发生
打开阅读框
基因组
诱导多能干细胞
遗传学
转基因
细胞生物学
肽序列
胚胎干细胞
作者
Garry A. Luke,Martin D. Ryan
出处
期刊:Future Virology
[Future Medicine]
日期:2013-10-01
卷期号:8 (10): 983-996
被引量:14
摘要
Synthetic biology enables us to create genes virtually at will. Ensuring that multiple genes are efficiently coexpressed within the same cell in order to assemble multimeric complexes, transfer biochemical pathways and transfer traits is more problematic. Viruses such as picornaviruses accomplish exactly this task: they generate multiple different proteins from a single open reading frame. The study of how foot-and-mouth disease virus controls its protein biogenesis led to the discovery of a short oligopeptide sequence, ‘2A’, that is able to mediate a cotranslational cleavage between proteins. 2A and ‘2A-like’ sequences (from other viruses and cellular sequences) can be used to concatenate multiple gene sequences into a single gene, ensuring their coexpression within the same cell. These sequences are now being used in the treatment of cancer, in the production of pluripotent stem cells, and to create transgenic plants and animals among a host of other biotechnological and biomedical applications.
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