期刊:International journal of systematic bacteriology [Microbiology Society] 日期:1978-10-01卷期号:28 (4): 616-620被引量:86
标识
DOI:10.1099/00207713-28-4-616
摘要
Serotypes B and C of Cryptococcus neoformans are described as a separate species, C. bacillisporus sp. nov. C. bacillisporus could be distinguished from C. neoformans by creatinine metabolism. Serotype B and C isolates utilized creatinine much more readily than A and D, resulting in increased ammonia production and alkaline pH. Based on these findings, a creatinine-dextrose-bromothymol blue agar medium (pH 5.7) was developed as a single-step culture method for separation of the two species. Isolates of C. neoformans can be distinguished from C. bacillisporus with 97% accuracy by 48 h.