Sulforaphene, an isothiocyanate present in radish plants, inhibits proliferation of human breast cancer cells

克隆形成试验 细胞凋亡 活力测定 癌细胞 自噬 细胞周期 氧化应激 程序性细胞死亡 异硫氰酸盐 细胞周期检查点 癌症研究 化学 细胞生物学 生物 癌症 生物化学 遗传学
作者
Anna Pawlik,Marta Wała,Aleksandra Hać,Agnieszka Felczykowska,Anna Herman-Antosiewicz
出处
期刊:Phytomedicine [Elsevier]
卷期号:29: 1-10 被引量:41
标识
DOI:10.1016/j.phymed.2017.03.007
摘要

Isothiocyanates derived from the Brassicaceae plants possess chemopreventive and anticancer activities. One of them is sulforaphene (SF), which is abundant in Rhapanus sativus seeds. The underlying mechanism of its anticancer activity is still underexplored.SF properties make it an interesting candidate for cancer prevention and therapy. Thus, it is crucial to characterize the mechanism of its activity.We investigated the mechanism of antiproliferative activity of SF in breast cancer cells differing in growth factor receptors status and lacking functional p53.Viability of SKBR-3 and MDA-MB-231 breast cancer cells treated with SF was determined by SRB and clonogenic assays. Cell cycle, cell death and oxidative stress were analyzed by flow cytometry or microscopy. The levels of apoptosis and autophagy markers were assessed by immunoblotting.SF efficiently decreased the viability of breast cancer cells, while normal cells (MCF10A) were less sensitive to the analyzed isothiocyanate. SF induced G2/M cell cycle arrest, as well as disturbed cytoskeletal organization and reduced clonogenic potential of the cancer cells. SF induced apoptosis in a concentration-dependent manner which was associated with the oxidative stress, mitochondria dysfunction, increased Bax:Bcl2 ratio and ADRP levels. SF also potentiated autophagy which played a cytoprotective role.SF exhibits cytotoxic activity against breast cancer cells even at relatively low concentrations (5-10µM). This is associated with induction of the cell cycle arrest and apoptosis. SF might be considered as a potent anticancer agent.
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