多酚
鞣花单宁
新陈代谢
化学
胃肠道
肠道菌群
可水解单宁
微生物代谢
生物利用度
生物化学
丙酸盐
食品科学
生物
抗氧化剂
细菌
药理学
遗传学
作者
Rocío Garcı́a-Villalba,Hanne Vissenaekens,Judit Pitart,María Romo‐Vaquero,Juan Carlos Espı́n,Charlotte Grootaert,María V. Selma,Katleen Raes,Guy Smagghe,Sam Possemiers,John Van Camp,Francisco A. Tomás‐Barberán
标识
DOI:10.1021/acs.jafc.7b02049
摘要
A TWIN-SHIME system was used to compare the metabolism of pomegranate polyphenols by the gut microbiota from two individuals with different urolithin metabotypes. Gut microbiota, ellagitannin metabolism, short-chain fatty acids (SCFA), transport of metabolites, and phase II metabolism using Caco-2 cells were explored. The simulation reproduced the in vivo metabolic profiles for each metabotype. The study shows for the first time that microbial composition, metabolism of ellagitannins, and SCFA differ between metabotypes and along the large intestine. The assay also showed that pomegranate phenolics preserved intestinal cell integrity. Pomegranate polyphenols enhanced urolithin and propionate production, as well as Akkermansia and Gordonibacter prevalence with the highest effect in the descending colon. The system provides an insight into the mechanisms of pomegranate polyphenol gut microbiota metabolism and absorption through intestinal cells. The results obtained by the combined SHIME/Caco-2 cell system are consistent with previous human and animal studies and show that although urolithin metabolites are present along the gastrointestinal tract due to enterohepatic circulation, they are predominantly produced in the distal colon region.
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