生物
绿色荧光蛋白
拟南芥
薄层荧光显微镜
共焦显微镜
多光谱图像
细胞生物学
蛋白质亚细胞定位预测
自体荧光
分生组织
荧光寿命成像显微镜
显微镜
活体细胞成像
荧光显微镜
荧光
荧光蛋白
基因
细胞
遗传学
物理
量子力学
突变体
光学
标识
DOI:10.1016/s0091-679x(08)61953-6
摘要
Intercellular communication plays a coordinating role in the development of all multicellular organisms. This chapter uses the Arabidopsis thaliana root meristem as a model system for investigating intercellular interactions. Arabidopsis is amenable to genetic manipulation, and one can routinely generate transgenic lines for work with the intact organism. The chapter adapts the jellyfish green fluorescent protein (GFP) for use as a directly visible gene marker in Arabidopsis and develops genetic and optical techniques to visualize and manipulate cells within living plants. Unlike enzyme markers, GFP can be visualized at high resolution in living cells using confocal microscopy. The images are not prone to fixation or staining artifacts, and can be of exceptional clarity. Moreover, the activities of living cells, such as cytoplasmic streaming, are clearly evident during microscopy. Ordinarily, movement within a sample is a nuisance, placing constraints on the use of sometimes lengthy techniques for noise reduction during confocal microscopy, such as frame averaging. However, it is possible to monitor dynamic events by time-lapse confocal microscopy, and this combination of a vital fluorescent reporter with high-resolution optical techniques shows much promise for use in cell biological and physiological experiments.
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