酪氨酸
连二亚硫酸钠
硝基酪氨酸
化学
生物化学
氨基酸
四硝基甲烷
二硫苏糖醇
连二亚硫酸钠
酶
有机化学
一氧化氮合酶
作者
Frank J. Dekker,Nicolas Abello,Rosalina Wisastra,Rainer Bischoff
标识
DOI:10.1002/0471140864.ps1413s69
摘要
Nitrotyrosine is a post-translationally modified amino acid with distinctly different properties than tyrosine or any other of the genetically encoded amino acids. Detecting proteins containing nitrotyrosine is the first step towards a better understanding of the role of nitrotyrosine in health and disease. Moreover, quantifying the extent of nitrotyrosine and determining its location in a protein forms the basis for a better understanding of the effect of tyrosine nitration on biological function. Described in this unit is a method to detect tyrosine-nitrated proteins in tissue sections and on western blots after creating a fluorescent complex between aminotyrosine, salicylaldehyde, and Al(3+). In addition, an approach is detailed for labeling aminotyrosine with biotin to enrich peptides from complex samples. Both methods require reduction of nitrotyrosine to aminotyrosine, which can be achieved with sodium dithionite or hemin plus dithiothreitol.
科研通智能强力驱动
Strongly Powered by AbleSci AI