[Feasibility of echocardiography-guided repeated intraventricular blood sampling in mice].

Objective: To investigate the feasibility of echocardiography-guided closed-chest repeated intraventricular blood sampling in mice, and to clarify the maximum blood volume that can be collected by this method, and whether the method can be used for long-term repeated blood collection in mice. Methods: Twenty-four male C57BL/6J mice (10-14 weeks old) were divided into the terminal experiment group (n=4, for investigating the maximum blood amount that could be sampled at one time), the repeated 0.5 ml blood collection group (n=10, sampling 0.5 ml whole blood each time, once every two days for consecutive 4 weeks), and the repeated 0.75 ml blood collection group (n=10, sampling 0.75 ml whole blood each time, once every two days for consecutive 4 weeks). High-frequency echocardiography was used to display the largest section of the left ventricle, guiding the insulin syringe needle through the thorax into the left ventricle for blood collection. In the repeated 0.5 ml blood collection group, echocardiography was used to detect the cardiac structure and function before blood collection, three minutes after blood collection, and one week after the last (the 14th) blood collection. Results: We successfully performed echocardiography-guided closed-chest intraventricular blood sampling, with an average operating time (88±19)s per mouse, and a maximum blood volume (1.43±0.11)ml per mouse. In the repeated 0.5 ml blood collection group, heart rate, left ventricular ejection fraction, left ventricular fractional shortening, left ventricular end-diastolic dimension and left ventricular posterior wall end-diastolic thickness remained uncganged before the first blood collection and after 4 weeks of repeated blood collection (all P>0.05). No death in the repeated 0.5 ml blood collection group. However, in the 0.75 ml blood collection group, two mice died before the end point. Conclusions: The echocardiography-guided closed-chest intraventricular blood sampling is a safe, minimally invasive, convenient and efficient method, and can be used repeatedly for long-term blood collection in mice.目的： 探索超声引导下不开胸进行小鼠心脏精准穿刺取血的方法，以明确该方法所能采集的最大血量，以及是否可以用于长期重复取血。 方法： 将雄性C57BL/6J小鼠（10~14周龄）分为终止性实验组（n=4，检测一次所能取到的最大血量）、反复采血0.5 ml组（n=10，每次取0.5 ml全血，1次/2 d，持续4周），反复采血0.75 ml组（n=10，每次取0.75 ml全血，1次/2 d，持续4周）。使用高频心脏超声显示左心室最大切面，引导胰岛素注射器针头经胸廓进入左心室进行采血。在反复采血0.5 ml组，使用超声检测第一次采血前、第一次采血后3 min、以及采血结束（共采血14次）休息1周后的心脏结构和心功能变化。 结果： 成功施行超声引导下不开胸经心脏穿刺取血，用时（88±19）s/只，获取的最大血量为（1.43±0.11）ml/只。在反复采血0.5 ml组，反复采血4周后，小鼠的心率、左心室射血分数、左心室缩短分数、左心室舒张末期内径及左心室舒张末期后壁厚度与第1次采血前比较，差异无统计学意义（P均>0.05），无小鼠死亡。但在反复采血0.75 ml组，在观察终点前有2只小鼠死亡。 结论： 在超声引导下行小鼠心脏穿刺取血，安全、微创、便捷、高效，可获取血量大，也可用于长期多次取血。.