流式细胞术
染色
骨髓
病理
电池类型
细胞
生物
脾脏
细胞生物学
细胞仪
巨噬细胞
分子生物学
化学
免疫学
医学
体外
生物化学
作者
Jacqueline K. Flynn,Nadia S. Deen,James Harris
出处
期刊:Methods in molecular biology
日期:2019-11-19
卷期号:: 57-66
被引量:2
标识
DOI:10.1007/978-1-4939-9936-1_6
摘要
Phenotyping cells by flow cytometry is a powerful way to identify cell type and any morphological changes during cell culture. The staining procedure used in this chapter enables the characterization of mouse macrophages by a flow cytometry antibody panel which can be used for both bone marrow-derived macrophages (BMM) and macrophages derived from other tissues, such as the mouse spleen or peritoneal cavity. The surface and intracellular staining methods are versatile and can be applied to flow cytometry staining of several different cell types by changing the surface markers used with knowledge of which receptors are expressed on different cell types.
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