免疫系统
固有层
肠道相关淋巴组织
肠道通透性
内科学
上皮内淋巴细胞
内分泌学
肠癌
回肠
小肠
炎症
生物
肠道菌群
脂肪酸
多不饱和脂肪酸
化学
肠粘膜
势垒函数
免疫学
胰岛素抵抗
结直肠癌
癌症
医学
上皮
遗传学
作者
Shohei Tanaka,Yasuhiro Nemoto,Yuria Takei,Ryo Morikawa,Shigeru Oshima,Takashi Nagaishi,Ryuichi Okamoto,Kiichiro Tsuchiya,Terutaro Nakamura,Susanne Stutte,Mamoru Watanabe
标识
DOI:10.1016/j.bbrc.2019.11.158
摘要
In Japan and other Asian countries, increased fat uptake induced by a westernized diet is thought to be associated with an increased incidence of inflammatory bowel disease, colorectal cancer and food allergies; however, the mechanism for this remains unclear. High-fat diet (HFD)-fed mice are common animal models used to examine the effect of fat intake in vivo. HFDs are reported to exacerbate DSS-induced colitis and intestinal tumorigenesis, but the effect of HFDs on the intestines before disease induction is often overlooked. We found that the intestinal and gut-associated lymphoid tissue (GALT) morphology of HFD-fed mice differed from that of standard diet (SD)-fed mice. To clarify the mechanism by which fat intake increases intestinal diseases, we analyzed the morphological and immunological aspects of the intestines of HFD-fed mice as well as the molecular mechanisms and physiology. Feeding an HFD for 3 weeks induced atrophy of the small intestine, colon and GALT and reduced the number of small intestinal intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs). Feeding an HFD for only one day reduced the number of small intestinal (SI)-IELs and SI-LPLs. The effect of feeding a 3-week HFD continued for 2 weeks after returning to the SD. The effect of the HFD on the intestinal immune system was independent of the gut microbes. We hypothesized that the cytotoxicity of the abundant HFD-derived free fatty acids in the intestinal lumen impairs the intestinal immune system. Both saturated and unsaturated free fatty acids were toxic to intestinal T-cells in vitro. Orally administering free fatty acids reduced the number of SI-IELs and LPLs. Using a lipase inhibitor to reduce the luminal free fatty acids attenuated the HFD-induced changes in the intestinal immune system, while using a statin to reduce the serum free fatty acids did not. Thus, HFD-induced free fatty acids damaged the intestines; this effect was termed “intestinal lipotoxicity”. Because sustained reduction of SI-LPLs after HFD feeding exacerbated indomethacin-induced small intestinal damage, lipotoxicity to the human intestines incurred by consuming a westernized diet in Japan may increase intestinal diseases such as IBD, colorectal cancer or food allergies. • Feeding a high-fat diet (HFD) induced atrophy of the intestines. • Feeding an HFD reduced the number of small intestinal T-cells, too. • We defined these phenotypes as termed intestinal lipotoxicity. • Intestinal lipotoxicity was caused by cytotoxicity from HFD-derived free fatty acids. • Intestinal lipotoxicity exacerbated indomethacin-induced small intestinal damage.
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