Abstract In this study, deflection values occurring on the main girder of a portal crane at various payloads were investigated theoretically, numerically and experimentally. The entire portal crane structure was analyzed on via a computer model in order to assess the reliability of the main girder in terms of deflection. Variations in deflection on the main girder were determined in accordance with load lifted and the crane trolley position. In addition, deflection values at critical points on the main girder of the portal crane were measured experimentally. Results indicate that maximum deflection value occurring on the main girder when the crane trolley was located at the middle of main girder were 1.34 times greater than the deflection value when the crane trolley was located at loading position 3.