First Report of Fusarium brachygibbosum Causing Root Rot on Soybean in Northeastern China

HomePlant DiseaseVol. 105, No. 5First Report of Fusarium brachygibbosum Causing Root Rot on Soybean in Northeastern China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Fusarium brachygibbosum Causing Root Rot on Soybean in Northeastern ChinaS. Wang, X. Li, C. Liu, L. Liu, F. Yang, Y. Guo, and Y. LiS. Wanghttps://orcid.org/0000-0002-8419-7415Plant Protection Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China, X. Li†Corresponding author: X. M. Li; E-mail Address: xinmin63@163.comPlant Protection Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China, C. LiuPlant Protection Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China, L. LiuPlant Protection Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China, F. YangPlant Protection Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China, Y. GuoJiusan Grain and Oil Industry Group Co. Ltd., Harbin 150060, China, and Y. LiAgricultural College, Northeast Agricultural University, Harbin 150030, ChinaAffiliationsAuthors and Affiliations S. Wang1 X. Li1 † C. Liu1 L. Liu1 F. Yang1 Y. Guo2 Y. Li3 1Plant Protection Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China 2Jiusan Grain and Oil Industry Group Co. Ltd., Harbin 150060, China 3Agricultural College, Northeast Agricultural University, Harbin 150030, China Published Online:7 Apr 2021https://doi.org/10.1094/PDIS-05-20-0941-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleIn August 2017, soybean plants exhibiting root rot were observed in Baiquan County (47°60′N, 126°10′E), Heilongjiang province, China. The disease occurred on approximately 65% of soybean (cv. Heihe43) plants in five fields (>10 ha). The disease resulted in yellowing or wilting and smaller sized leaves, absence of lateral roots, and black lesions on tap roots. Infected root tissues from 10 individual plants (two plants per field) were surface disinfested with 0.5% NaOCl for 2 min, rinsed three times in sterile distilled water, placed on potato dextrose agar (PDA), and incubated at 26°C for 3 days. Eight fungal isolates were obtained by transferring hyphal tips. Colonies on PDA were initially white to rose and then yellow in color with abundant aerial mycelium. The fungal colonies grew to a size of 7.4 cm in diameter 4 days after inoculation. Macroconidia were scarce and scattered, measuring 19.7 × 3.5 μm (n = 50) on carnation leaf agar. Typical macroconidium had three to five septa, slightly sharp apices with a distinct basal foot cell. Microconidia had zero to two septa and were slightly curved, measuring 10.7 × 3.2 μm (n = 50). Spherical chlamydospores had a mean diameter of 13.7 μm (n = 50) and were terminal and intercalary on PDA. According to these morphological characteristics, the fungus was identified as Fusarium brachygibbosum (Padwick 1945). Genomic DNA of a representative isolate (P13-1) was extracted. The Ef-1α, RPB1, and RPB2 regions were amplified using primers ef1/ef2, Fa/G2R, and 5f2/7cr (O'Donnell et al. 2010). The consensus sequences (accession nos. MH748277, MH748278, and MH748279) showed 98.65, 98.91, and 99.54% identity to the sequences of F. brachygibbosum strain NRRL 34033 (accession nos. GQ505418.1, HM347172.1, and GQ505482.1). Isolate P13-1 was preserved in the Agricultural Culture Collection of China, and stock ID number is ACCC 39715. To confirm pathogenicity of P13-1, soybean (cv. Heihe43) seeds were grown in 15-cm pots containing a commercial potting mix (five seeds per pot, three pots per treatment). Sorghum seeds (10 g) fully colonized by F. brachygibbosum (Li et al. 2018) were uniformly distributed in each pot and then covered with a 0.5-cm layer of sterile potting soil, sterilized sorghum seeds (10 g) were added to control pots, and pots were incubated in a growth chamber at 25°C (12-h day)/20°C (12-h night). Ten days after inoculation, all inoculated plants showed symptoms consistent with those observed in the fields. The experiment was repeated two times. F. brachygibbosum was reisolated from diseased plants and identified as F. brachygibbosum based on morphological and gene sequences analysis. No fungal pathogens were isolated from nontreated controls. To our knowledge, this is the first report of F. brachygibbosum on soybean in China. The soybean is the prime oil seed crop and the source of protein cultivated in Northeast China, and this disease seriously affects the seedling growth. So, our findings are very important for the establishment of control strategies and breeding for resistance to soybean root rot.The author(s) declare no conflict of interest.References:Li, Y. G., et al. 2018. J. Agric. Sci. 10:13. Google ScholarO'Donnell, K., et al. 2010. J. Clin. Microbiol. 48:3708. https://doi.org/10.1128/JCM.00989-10 Crossref, ISI, Google ScholarPadwick, G. W. 1945. Mycol. Pap. 12:1. Google ScholarThe author(s) declare no conflict of interest.Funding: This research was funded by Soybean Industrial Technology Collaborative Innovation System of Heilongjiang Province of China.DetailsFiguresLiterature CitedRelated Vol. 105, No. 5 May 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms of bacterial leaf spot on Cucurbita pepo leaf 5 days after spray inoculation (L. Li et al.). Photo credit: B. J. Li. Symptoms of Fusarium wilt on cyclamen (V. Guarnaccia et al.). Photo credit: M. L. Gullino. Metrics Article History Issue Date: 26 Aug 2021Published: 7 Apr 2021First Look: 6 Jan 2021Accepted: 5 Jan 2021 Page: 1560 Information© 2021 The American Phytopathological SocietyFundingSoybean Industrial Technology Collaborative Innovation System of Heilongjiang Province of ChinaKeywordsFusarium spp.soybean diseaseHeilongjiangFusarium brachygibbosumfungiThe author(s) declare no conflict of interest.PDF downloadCited byGenome Sequence Resource of Fusarium brachygibbosum Padwick Strain HN-1, a Causal Agent of Maize Stalk Rot DiseaseHafiz Abdul Haseeb, Sajjad Hyder, Meixu Gao, and Wei Guo20 January 2022 | Plant Disease, Vol. 106, No. 1Pest categorisation of Fusarium brachygibbosumEFSA Journal, Vol. 19, No. 11