Antioxidant activity and synergism of canolol and α-tocopherol in rapeseed oil is affected by the presence of phospholipid association colloids

Interactions of canolol and α-tocopherol (α-T) with reverse micelles made from DOPC phospholipid (1,2-dioleoyl-sn-glycero-3-phosphocholine) in rapeseed oil were investigated in the current study. Furthermore, the antioxidant efficiency of canolol and α-T as well as the phenomenon of synergism between them in the oil containing association colloids has been determined. As demonstrated by fluorescence measurements, canolol may be incorporated into the structure of DOPC reverse micelles. This antioxidant may also accumulate in bulk oil at oil/water interface. In turn, no interactions of α-T with phospholipid association colloids were observed. The presence of DOPC association colloids affects antioxidant efficiency of canolol and α-T. Simultaneously, at the concentration 100 μmol of both substances, decreasing of their antioxidant activity effectiveness over time was observed. Formed during autoxidation amphiphilic compounds probably influence the structure of reverse micelles, modifying effectiveness of antioxidants. Antioxidant synergism of canolol and α-T occurs only in DOPC reverse micelles. Synergism phenomenon allows eliminating adverse effect of autoxidation products on the effectiveness of antioxidants. The presence of canolol may eliminate prooxidative action of α-T.