平移(音频)
噬菌体展示
噬菌体
克隆(编程)
抗体
单域抗体
生物
计算生物学
计算机科学
遗传学
分子生物学
噬菌体
基因
大肠杆菌
缩放
程序设计语言
古生物学
镜头(地质)
作者
Carolin Sellmann,Lukas Pekar,Christina Bauer,Elke Ciesielski,Simon Krah,Stefan Becker,Lars Toleikis,Jonas Kügler,André Frenzel,Bernhard Valldorf,Michael Hust,Stefan Zielonka
标识
DOI:10.1007/s12033-020-00236-0
摘要
In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. We show that single chain variable fragment (scFv) libraries with adequate qualities can readily be cloned in a 'scar-less' manner and that the isolation of antigen-specific antibodies from immunized chickens is feasible within three selection rounds. Moreover, we demonstrate the general applicability of this method by rapidly constructing and panning VHH single domain antibody phage display libraries from immunized Llama repertoires.
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