Anesthetic Effects of Three Kind of Narcotic Drugs on Mice

Objective To find a suitable anesthesia for C57BL/6J mice,the anesthesia effects of tribromoethanol,chloral hydrate and urethane were compared.Method The three anaesthetic were processed intraperitoneal injection with different dose gradients respectively,and then the anesthesia onset time,depth,duration and recovery time were compared.Result Tribromoethanol(1.2%) at dosage of 0.60~0.65mL/20g and chloral hydrate(4%) at dosage of 0.20mL/20g showed the best anesthesia effect.But the urethane was not suitable for anaesthesia of C57BL/6J mice.Conclusion For anaesthesia of C57BL/6J mice,tribromoethanol(0.60mL/20g) and chloral hydrate(0.20mL/20g) have shortest anesthesia onset time,moderate anesthesia duration,adequate anesthesia depth and zero mortality rate by intraperitoneal injection.