OBJECTIVE To study the chemical constituents from Aeschynanthus longicaullis. METHOD The column chromatographic techniques were applied to isolate the constituents. The spectroscopie methods were used of EI-MS and NMR to identify the structures of the separated compounds. RESULT Seven compounds were isolated from the ethyl acetate extract of A. longicaullis, whose structures were elucidated as cryptomeridiol (1), 4 (15) -eudesmene-1beta, 6alpha-diol (2), 2,5-bornanediol (3), isovanillic acid (4), vanillic acid (5), stigmast-5 (6) , 22 (23)-diene-3beta-ol (6) and beta-sitosterol (7). CONCLUSION Compounds 1-5 were isolated for the first time from Gesneriaceae and 6-7 were isolated for the first time from this plant.