分子生物学
氯霉素乙酰转移酶
细胞周期蛋白依赖激酶
生物
增强子
转染
北方斑点
细胞周期蛋白依赖激酶2
G2水电站
激酶
CDK抑制剂
转录因子
细胞周期蛋白依赖激酶1
肝细胞
基因表达
报告基因
基因
细胞培养
生物化学
细胞周期
蛋白激酶A
遗传学
体外
作者
Daisaku Hida,Keisuke Nakata,Yuichi Shima,Kiyoshi Migita,K Nakao,Yasuhito Kato,N Ishii,K Eguchi
出处
期刊:PubMed
日期:1999-01-19
卷期号:18 (6A): 4317-22
被引量:5
摘要
Butyrolactone I is a selective inhibitor of the cyclin-dependent kinase (cdk) family, cdk2 and cdc2 kinase. In the present study, the effect of butyrolactone I on expression of the albumin and alpha-fetoprotein (AFP) genes was investigated in HuH-7 human hepatoma cells. Butyrolactone I inhibited cell growth and arrested cells predominantly in G2/M phase. By Northern blot analysis, the levels of both albumin and AFP mRNA were suppressed dose-dependently by butyrolactone I. In transient chloramphenicol acetyltransferase plasmid transfection experiments, the albumin promoter activity and the AFP promoter and enhancer activities were suppressed by butyrolactone I. Consistent with this, the transcripts of hepatocyte nuclear factor-1 (HNF-1), a liver-specific transcription factor which transactivates these promoter and enhancer regions were reduced by butyrolactone I in a dose-dependent manner. These results indicate that butyrolactone I down-regulates both the albumin and the AFP gene transcription through the reduction of HNF-1 expression.
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