Study on Extraction and Purification of Polysaccharides from Cordyceps militaris

The medium mainly containing rice and the pupa of silkworm was used to cultivate the sporocarp of Cordyceps militaris.Extraction processing of water-soluble polysaccharide in Cordyceps militaris was studied through orthogonal experiment,and the anthrone-sulphuric acid method was used to determine polysaccharide content.Three times(3 hours per times) extraction with 20 times water at 90 ℃ was the best extraction conditions.Simultaneously deproteinization effect was examined with different methods.