Comparison of two recurrent Aphthous ulcer models in SD rats

Objective: To compare the effect of two recurrent aphthous ulcer(RAU)models in SD rats.Methods: RAU models were established by injecting the mixture of homogenate tissue and complete Freund's adjuvant under oral mucosal of SD rats(immunization group) and by burning method with acetic acid(burning group) respectively.Normal healthy rats were used as the controls.The weight and food consumption of the rats in each group were detected,and histopathology examination was performed for mucosa ulcer.The number of peripheral T-lymphocytes subsets in circulating blood was tested by FCM.Results: All of the experimental rats developed oral ulcer.There was significantly difference in the weight and food consumption between control and experimental groups(P0.05).In immunization group,numbers of CD3+CD4+and the ratio of CD4+ to CD8+ T-lymphocyte subsets were lower than that in control group(P0.05).In burning group,numbers of CD3+CD8+ T-lymphocyte subsets showed no difference compared with the controls(P0.05).Conclusion: The two RAU models of SD rats were similar to RAU in human beings clinically and pathologically.The occurrence of RAU of rats was related with food consumption weight of rats and the ratio of CD4+ to CD8+ T lymphocyte subsets.