A cheaper substitute for HRP: ultra-small Cu–Au bimetallic enzyme mimics with infinitesimal steric hindrance to promote catalytic lateral flow immunodetection of clenbuterol

A lateral flow immunoassay (LFIA) is developed for enzyme-catalyzed double-reading determination of clenbuterol (CLE) through the direct electrostatic adsorption of ultra-small copper–gold bimetallic enzyme mimics (USCGs) and monoclonal antibodies.