脱质子化
荧光
水杨醛
化学
结合常数
氢键
密度泛函理论
酰肼
转身(生物化学)
分析化学(期刊)
质子核磁共振
离子
化学计量学
选择性
检出限
分子
物理化学
结晶学
计算化学
立体化学
结合位点
催化作用
席夫碱
有机化学
色谱法
量子力学
生物化学
物理
作者
Hardeep Kaur,. Riya,Amandeep Singh,Harpreet Singh,Uma Ranjan Lal,Ashutosh Kumar,M.V.N.L. Chaitanya
标识
DOI:10.1016/j.saa.2023.123270
摘要
A novel turn-on fluorescent probe 3 was synthesized by condensing salicylaldehyde and nicotinic hydrazide for the selective detection of CO32- in aqueous medium. Probe 3 exhibited a turn-on fluorescence response toward CO32- with excellent selectivity, sensitivity (DL = 2.76 μM), and good reversibility. The binding constant (K) of probe 3 with CO32- was calculated to be 5×103 M−1 (log K 3.69). The 1:1 stoichiometry of the complex between probe 3 and CO32- ions was confirmed by Job's plot and ESI-MS spectra. Deprotonation and hydrogen-bonding interactions are involved in the recognition of CO32- ion, which was also suggested by 1H NMR, ESI-MS spectra, and Density Functional Theory (DFT) calculations. Moreover, an INHIBIT type molecular logic gate was constructed by using 3:CO32- and CH3COOH as inputs and current signal as output. Owing to the practical applications, probe 3 demonstrated its efficiency in quantifying CO32- ion in real water samples through standard addition method, thus showcasing its potential in real environment. Further, the MTT assay indicated very low cytotoxicity (IC50 =1mM) of probe 3 and also the cell imaging experiments demonstrated the effective sensing of CO32- ions with probe 3 in the biological systems.
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