Identification and Localization of Cell Types in the Mouse Olfactory Bulb Using Slide-SeqV2

Spatial transcriptomics maps RNA Cell typesmolecules Miceto Olfactory bulbsthe location in a tissue where they are expressed. Here we document the use of Slide-SeqV2 to visualize gene expression in the mouse olfactory bulb (OB). This approach relies on spatially identified beads to locate and quantify individual transcripts. The expression profiles associated with the beads are used to identify and localize individual cell types in an unbiased manner. We demonstrate the various cell types and subtypes with distinct spatial locations in the olfactory bulb that are identified using Slide-SeqV2.