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Impaired S-nitrosylation of Cx43 prevents arrhythmogenicity and myocardial injury upon cardiac stress in Duchenne Muscular Dystrophy

杜氏肌营养不良 肌营养不良 医学 心脏病学 内科学
作者
Manuel F. Muñoz,Jonathan Quan,Thao T Nguyen,Janet Nuno,Alexander Sheehy,Pía C. Burboa,Pablo S. Gaete,Mauricio A. Lillo,Jorge E. Contreras
标识
DOI:10.1101/2024.08.29.610357
摘要

Connexin-43 (Cx43) plays a critical role in the propagation of action potentials and cardiac contractility. In healthy cardiomyocytes, Cx43 is mainly located at the intercalated disk; however, Cx43 remodeling is observed in cardiac pathologies and is linked with arrhythmogenesis and sudden cardiac death. Using a mouse model of Duchenne muscular dystrophy (DMD), we previously demonstrated that Cx43 localizes to the lateral side of dystrophic cardiomyocytes, forming undocked hemichannels. β-adrenergic signaling-induced cardiac stress promotes S-nitrosylation and the opening of undocked Cx43 hemichannels leading to disrupted cardiac membrane excitability and deadly arrhythmogenic behaviors. To establish the direct role of S-nitrosylated Cx43 in DMD cardiomyopathy, we generated knockin DMD mdx mice with reduced levels of S-nitrosylated Cx43, by replacing cysteine 271 with a serine in one Cx43 of the unique site for S-nitrosylation of Cx43 (DMD mdx :C271S +/- ). Immunofluorescence analysis revealed that cardiac Cx43 lateralization in DMD mdx :C271S +/- mice was similar to DMD mdx mice, indicating that the genetic modification did not prevent Cx43 remodeling. Upon isoproterenol treatment, DMD mdx mice displayed a higher incidence of arrhythmogenic events when compared to DMD mdx :C271S +/- mice, which more closely resemble wild-type mice. Optical mapping imaging in isolated hearts showed that DMD mdx mice displayed aberrant Ca 2+ signaling and prolonged action potentials, which is restored in DMD mdx :C271S +/- mice. Isoproterenol treatment evoked severe myocardial injury in DMD mdx mice, which was significantly attenuated in DMD mdx :C271S +/- mice. Notably, DMD mdx mice treated with Gap19, a Cx43 hemichannel blocker, exhibited cardioprotection against myocardial injury. We concluded that S-nitrosylation of Cx43 proteins is a fundamental NO-mediated mechanism involved in arrhythmias and myocardial injury in DMD mdx , occurring through the opening of hemichannels following β-adrenergic stress.

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