Molecular evidence reveals fish-pathogenic Aphanomyces spp. (Oomycetes: Saprolegniales) on new host of the threatened seasonal cyprinodont fish Nothobranchius spp. (Teleostei: Cyprinodontiformes) in south-eastern Democratic Republic of Congo

AbstractThe Aphanomyces species pose an important global threat and cause damage to aquaculture and agriculture by infecting aquatic animals and plants. Several subpopulations of the seasonal cyprinodont fish genus Nothobranchius with lesions suggestive of infection with Aphanomyces invadans, were observed in ephemeral wetland habitats of the upper Congo drainage in the south-eastern Democratic Republic of Congo. Molecular analyses on samples collected in 2023 revealed evidence of fish-pathogenic oomycetes Aphanomyces invadans and A. laevis, for the first time in the host of fish genus Nothobranchius spp. All Nothobranchius fishes in the upper Congo drainage are subject to a high level of threat and belong to one of the threatened Red List categories, due to habitat degradation of seasonal wetlands. These fishes complete their seasonal life cycle in ephemeral natural habitats. The latter makes them highly vulnerable, as such wetland habitats are often degraded by the cumulative effect of multiple human stressors which include: the cultivation of wetlands for agriculture, the abstraction of water, the expansion of urban areas and the pollution load. Evidence of infection by oomycete pathogens documented in this study, represents a critical additional threat to the unique seasonal aquatic biodiversity of the region.Keywords: barcodingbiodiversity threatCongo drainageepizootic ulcerative syndromephylogeny