Electrochemical detection mechanism of estrogen effect induced by cadmium: The regulation of purine metabolism by the estrogen effect of cadmium

嘌呤代谢 黄嘌呤 化学 嘌呤 次黄嘌呤 尿酸 雌激素 新陈代谢 鸟嘌呤 从头合成 生物化学 雌激素受体 次黄嘌呤鸟嘌呤磷酸核糖转移酶 内分泌学 内科学 生物 核苷酸 医学 癌症 突变体 基因 乳腺癌
作者
Ying Wei,Hongkuan Shen,Changsheng Gao,Yuan Du,Yanli Zhao,Yuhang Wang,Shi Zhou,Jinlian Li,Baojiang Zhao,Dongmei Wu
出处
期刊:Chemosphere [Elsevier BV]
卷期号:311: 136970-136970 被引量:6
标识
DOI:10.1016/j.chemosphere.2022.136970
摘要

Some heavy metals in the environment may have estrogen-like activity, which probably lead to major diseases such as breast cancer. It is of great importance to establish new methods to evaluate the estrogen effect of heavy metals from multiple angles due to the complex mechanism of estrogen effect. In this paper, using MCF-7 cells as model, the electrochemical detection mechanism of the estrogen effect of heavy metal cadmium (Cd) was studied. The two electrochemical signals of MCF-7 cells derived from uric acid (0.30 V) and the mixture of guanine and xanthine (0.68 V) increased in a time and dose-dependent manner when MCF-7 cells induced by Cd, reaching the maximum at 96 h and 10-9 mol L-1. Further studies found that three purine metabolism pathways about de novo synthesis, salvage synthesis and decomposition metabolism were activated by the estrogen effect of Cd. The expression of PRPP amidotransferase in purine de novo synthesis pathway and HPRT in purine salvage synthesis pathway up-regulated, especially HPRT, which promoted cell proliferation together. Nevertheless, the expression of GDA and ADA, the key enzymes in purine decomposition metabolism pathway, up-regulated in a time and dose-dependent manner, which had same tendency with that of ERα, thereby increased the content of intracellular hypoxanthine, guanine, xanthine and uric acid, and enhanced electrochemical signals.
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