Jujuboside B inhibits febrile seizure by modulating AMPA receptor activity

AMPA受体 兴奋性突触后电位 海马结构 药理学 神经科学 癫痫 膜片钳 神经保护 医学 热性惊厥 电生理学 受体 谷氨酸受体 生物 内科学
作者
Baohua Jin,Wanjun Bai,Jiaojiao Zhao,Xia Qin,Han Guo,Yahui Li,Jie Hao,Siruan Chen,Zuxiao Yang,Hui Bai,Zongmao Zhao,Qingzhong Jia,Changzheng Dong,Zhuo Huang,Dezhi Kong,Wei Zhang
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:304: 116048-116048 被引量:4
标识
DOI:10.1016/j.jep.2022.116048
摘要

Febrile seizure is a common neurologic disorder with limited treatment occurring in infants and children under the age of five. Jujuboside B (JuB) is a main bioactive saponin component isolated from the Chinese anti-insomnia herbal medicine Ziziphi Spinosae Semen (ZSS), seed of Ziziphus jujuba Mill, which has been proved to exhibit neuroprotective effects recently.In this study, we aimed at elucidating the effect of JuB on suppressing febrile seizure and the potential mechanisms.Electroencephalogram (EEG) recording was used to monitor the severity of febrile seizures. The JuB in the brain was identified by mass spectrometry. Neuronal excitability was investigated using patch clamp.JuB (30 mg/kg) significantly prolonged seizure latency and reduced the severity in hyperthermia-induced seizures model mice. Hippocampal neuronal excitability was significantly decreased by JuB. And JuB significantly reduced the excitatory synaptic transmission mediated by α-amino-3-hydroxy-5-methyl-4-iso-xazolepropionic acid receptor (AMPAR), including evoked excitatory postsynaptic currents (eEPSCs), and miniature EPSCs (mEPSCs) in hippocampal neurons. Furthermore, JuB also significantly inhibited recombinant GluA1 and GluA2 mediated AMPA current in HEK293 cell and decreased the upregulation of [Ca2+]i induced by AMPA in primary cultured cortex neurons.JuB suppressed the excitability of hippocampal neurons by inhibiting the activity of AMPAR and reducing the intracellular free calcium, thereby relieving febrile seizures.
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