定向进化
合理设计
蛋白质工程
饱和突变
定向分子进化
突变
蛋白质设计
利用
计算机科学
计算生物学
酶
蛋白质结构
突变
生物
遗传学
生物化学
计算机安全
基因
突变体
作者
Manfred T. Reetz,Zhoutong Sun,Ge Qu
标识
DOI:10.1002/9783527836895.ch1
摘要
Directed evolution and rational design are the two main approaches in protein engineering, which were developed in the quest to solve the limitations of enzymes regarding insufficient catalytic properties. Directed enzyme evolution utilizes random introduction of mutations or focused mutations of the type combinatorial active-site saturation test (CAST)/iterative saturation mutagenesis (ISM), while rational design exploits structural and mechanistic information with computational aids. A panoramic view of directed enzyme evolution and rational enzyme design is given in this chapter, including the methods, aims, and historical developments up to the current status. The relative merits of the approaches are discussed.
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