Effects of culture conditions on the growth of the benthic dinoflagellates Ostreopsis cf. ovata, Prorocentrum lima and Coolia malayensis (Dinophyceae): A global review

Benthic dinoflagellates produce potent toxins that may negatively affect humans and the marine biota. Understanding the factors that stimulate their growth is important for management strategies and to reduce their potential negative impacts. Laboratory cultures have been extensively used to study microalgae physiology and characterize life cycles, nutrition, growth rates, among other processes. A systematic review of the literature on the growth parameters of the benthic dinoflagellates Ostreopsis cf. ovata, Prorocentrum lima species complex and Coolia malayensis obtained in laboratory cultures of strains isolated from all over the world was performed. The effects of temperature, light intensity, photoperiod, salinity and culture media on the growth rate of these species were evaluated using multiple regressions and a model selection approach, based on the Akaike Information Criteria (AIC). The potential effects of the initial culture abundance and the media volume used on the growth of the species were also assessed. Data from 50 articles (25 for O. cf. ovata, 21 for P. lima and 6 for C. malayensis), resulting in 399 growth parameter values (growth rate, doubling time and maximum yield) were compiled in a database. The genetic clades of O. cf. ovata and P. lima species complex were also noted. Growth rate was the most frequently reported growth parameter for the three species, and 127 values were retrieved for O. cf. ovata, 90 for P. lima and 56 for C. malayensis. Temperature was the factor that best explained the growth response of P. lima and C. malayensis, whereas for O. cf. ovata, temperature and salinity were equally important. Light intensity and photoperiod were included among the six best models for the studied species but presented a weaker effect on growth. Given the observed and future projected climate change, increasing ocean temperature will promote the growth of these species, likely leading to an expansion of their impacts on ecosystems and human health. The use of common garden experiments using multiple strains from different geographic domains, particularly addressing underrepresented lineages is recommended, as they will provide more balanced insight regarding the species physiological responses to environmental drivers.