cccDNA
核仁素
乙型肝炎病毒
生物
微小染色体
病毒学
病毒复制
抄写(语言学)
癌症研究
病毒
DNA
细胞生物学
染色质
遗传学
乙型肝炎表面抗原
细胞质
核仁
哲学
语言学
作者
Yuchen Xia,Xiaoming Cheng,Tobias Nilsson,Min Zhang,Gaihong Zhao,Tadashi Inuzuka,Yan Teng,Yao Li,David E. Anderson,Meghan Holdorf,T. Jake Liang
标识
DOI:10.1073/pnas.2306390120
摘要
Hepatitis B virus (HBV) remains a major public health threat with nearly 300 million people chronically infected worldwide who are at a high risk of developing hepatocellular carcinoma. Current therapies are effective in suppressing HBV replication but rarely lead to cure. Current therapies do not affect the HBV covalently closed circular DNA (cccDNA), which serves as the template for viral transcription and replication and is highly stable in infected cells to ensure viral persistence. In this study, we aim to identify and elucidate the functional role of cccDNA-associated host factors using affinity purification and protein mass spectrometry in HBV-infected cells. Nucleolin was identified as a key cccDNA-binding protein and shown to play an important role in HBV cccDNA transcription, likely via epigenetic regulation. Targeting nucleolin to silence cccDNA transcription in infected hepatocytes may be a promising therapeutic strategy for a functional cure of HBV.
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