Single-cell Transcriptome Landscape of DNA Methylome Regulators Associated with Orofacial Clefts in the Mouse Dental Pulp

DNA甲基化 DNA去甲基化 表观遗传学 生物 转录组 甲基化 去甲基化 染色质 甲基转移酶 牙髓干细胞 表观遗传学 遗传学 间充质干细胞 基因 基因表达
作者
Badam Enkhmandakh,Pujan Joshi,Paul Robson,Anushree Vijaykumar,Mina Mina,Dong‐Guk Shin,Dashzeveg Bayarsaihan
出处
期刊:The Cleft Palate-Craniofacial Journal [SAGE Publishing]
卷期号:61 (9): 1480-1492 被引量:2
标识
DOI:10.1177/10556656231172296
摘要

Objective Significant evidence links epigenetic processes governing the dynamics of DNA methylation and demethylation to an increased risk of syndromic and nonsyndromic cleft lip and/or cleft palate (CL/P). Previously, we characterized mesenchymal stem/stromal cells (MSCs) at different stages of osteogenic differentiation in the mouse incisor dental pulp. The main objective of this research was to characterize the transcriptional landscape of regulatory genes associated with DNA methylation and demethylation at a single-cell resolution. Design We used single-cell RNA sequencing (scRNA-seq) data to characterize transcriptome in individual subpopulations of MSCs in the mouse incisor dental pulp. Settings The biomedical research institution. Patients/Participants This study did not include patients. Interventions This study collected and analyzed data on the single-cell RNA expssion in the mouse incisor dental pulp. Main outcome measure(s) Molecular regulators of DNA methylation/demethylation exhibit differential transcriptional landscape in different subpopulations of osteogenic progenitor cells. Results scRNA-seq analysis revealed that genes encoding DNA methylation and demethylation enzymes (DNA methyltransferases and members of the ten-eleven translocation family of methylcytosine dioxygenases), methyl-DNA binding domain proteins, as well as transcription factors and chromatin remodeling proteins that cooperate with DNA methylation machinery are differentially expressed within distinct subpopulations of MSCs that undergo different stages of osteogenic differentiation. Conclusions These findings suggest some mechanistic insights into a potential link between epigenetic alterations and multifactorial causes of CL/P phenotypes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
丘比特应助科研通管家采纳,获得10
刚刚
刚刚
机灵柚子应助科研通管家采纳,获得10
刚刚
刚刚
科研通AI2S应助科研通管家采纳,获得10
刚刚
我是老大应助科研通管家采纳,获得10
1秒前
1秒前
1秒前
小蘑菇应助科研通管家采纳,获得10
1秒前
丘比特应助科研通管家采纳,获得10
1秒前
科研通AI6.3应助科研通管家采纳,获得100
1秒前
bkagyin应助科研通管家采纳,获得10
1秒前
传奇3应助芝衿采纳,获得10
1秒前
Ansong完成签到,获得积分10
2秒前
Alien发布了新的文献求助10
2秒前
2秒前
旺旺旺发布了新的文献求助20
3秒前
4秒前
4秒前
4秒前
研友_8QQlD8完成签到,获得积分20
4秒前
范小勤子发布了新的文献求助10
8秒前
CipherSage应助小歪采纳,获得10
8秒前
QQQQY发布了新的文献求助30
8秒前
stws完成签到,获得积分10
9秒前
han发布了新的文献求助10
9秒前
zhj发布了新的文献求助10
9秒前
10秒前
10秒前
11秒前
小涵砸发布了新的文献求助10
12秒前
14秒前
14秒前
柳白完成签到,获得积分20
14秒前
田园发布了新的文献求助10
14秒前
jingyuan发布了新的文献求助10
17秒前
17秒前
996完成签到,获得积分10
18秒前
18秒前
19秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Modern Epidemiology, Fourth Edition 5000
Digital Twins of Advanced Materials Processing 2000
Weaponeering, Fourth Edition – Two Volume SET 2000
Polymorphism and polytypism in crystals 1000
Signals, Systems, and Signal Processing 610
Discrete-Time Signals and Systems 610
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 纳米技术 有机化学 物理 生物化学 化学工程 计算机科学 复合材料 内科学 催化作用 光电子学 物理化学 电极 冶金 遗传学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 6025210
求助须知:如何正确求助?哪些是违规求助? 7660817
关于积分的说明 16178551
捐赠科研通 5173359
什么是DOI,文献DOI怎么找? 2768159
邀请新用户注册赠送积分活动 1751580
关于科研通互助平台的介绍 1637661