萃取(化学)
双肌酸测定
蛋白质纯化
化学
色谱法
产量(工程)
微波食品加热
材料科学
冶金
计算机科学
电信
作者
Qianyi Wu,Katelyn A. Congreves,Richard E. Farrell
出处
期刊:Canadian Journal of Soil Science
[Canadian Science Publishing]
日期:2023-12-01
卷期号:103 (4): 684-688
标识
DOI:10.1139/cjss-2023-0016
摘要
Soil protein is an important indicator of soil health and for soil health assessments is usually determined using autoclaved citrate extraction (ACE) followed by protein quantification using the Bradford or bicinchoninic acid assay. Here, we investigated an alternative extraction process using microwave-assisted citrate extraction. We show that protein yield increases as the extraction time increases, but that yields comparable to those obtained using the standard ACE method can be obtained with an extraction time as short as 15 min. To the best of our knowledge, this is the first report of microwave-assisted extraction being used to determine this soil protein pool.
科研通智能强力驱动
Strongly Powered by AbleSci AI