电穿孔
清脆的
有机体
微生物
质粒
基因
计算生物学
转化(遗传学)
基因组编辑
生物
生化工程
工业微生物学
Cas9
工业生物技术
水平基因转移
拉伤
生物技术
遗传学
基因组
细菌
工程类
解剖
作者
Sayani Ghosh,P Pooja,Supratim Datta
标识
DOI:10.1007/978-981-99-2816-3_17
摘要
Microorganisms are subjected to strain improvement techniques to improve the performance of metabolites produced and increase the efficiency of existing enzymes and pathways that produce compounds of industrial relevance. Strain improvement techniques are used to modify native pathways or to explore previously unknown properties of a non-model organism to synthesize the product of our interest. Recombinant DNA technology is a standard method for introducing desired mutations through plasmid transfer via techniques like transformation or electroporation into the host organism. These microorganisms are introduced with foreign properties by vital genetic manipulations like gene deletion, gene insertion, or overexpression to produce metabolites of industrial interest. Modern-day strategies like the CRISPR/Cas9 gene editing technique are excellent methods for performing genetic modifications in such cases. In this chapter, we elucidate the various strategies used to introduce gene modifications in these industrially relevant microorganisms toward the proper utilization of natural resources for the betterment of our society.
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