Spray Vaccination with a Newcastle Disease Virus (NDV)-Vectored Infectious Laryngotracheitis (ILT) Vaccine Protects Commercial Chickens from ILT in the Presence of Maternally Derived Antibodies

新城疫 接种疫苗 病毒学 病毒 生物 效价 抗体 免疫 抗体效价 病毒释放 女主角 减毒疫苗 毒力 医学 免疫学 生物化学 基因
作者
Zhe Zeng,Zichen Wang,Xin Wang,Lun Yao,Yu Shang,Helong Feng,Hongcai Wang,Huabin Shao,Qingping Luo,Guoyuan Wen
出处
期刊:Avian Pathology [Informa]
卷期号:: 1-7
标识
DOI:10.1080/03079457.2024.2356676
摘要

Infectious laryngotracheitis (ILT) poses a significant threat to the poultry industry, and vaccines play an important role in protection. However, due to the increasing scale of poultry production, there is an urgent need to develop vaccines that are suitable for convenient immunization methods such as spraying. Previous studies have shown that Newcastle disease virus (NDV)-ILT vaccines administered via intranasal and intraocular routes to commercial chickens carrying maternally derived antibodies (MDAs) are still protective against ILT. In this study, a recombinant NDV (rNDV) was generated to express infectious laryngotracheitis virus (ILTV) glycoprotein B (gB), named rLS-gB, based on a full-length cDNA clone of the LaSota strain. The protective effect of different doses of rLS-gB administered by spray vaccination to commercial chickens at 1 day of age (DOA) was evaluated. The chickens were exposed to 160-μm aerosol particles for 10 min for spray vaccination, and no adverse reactions were observed after vaccination. Despite the presence of anti-NDV MDAs and anti-ILTV MDAs in chickens, the ILTV- and NDV-specific antibody titers were significantly greater in the vaccinated groups than in the unvaccinated group. After challenge with a virulent ILTV strain, no clinical signs were observed in the 107 EID50/ml group compared to the other groups. Furthermore, vaccination with 107 EID50/ml rLS-gB significantly reduced the ILTV viral load and ameliorated gross and microscopic lesions in the trachea of chickens. Overall, these results suggested that rLS-gB is a safe and efficient candidate spray vaccine for ILT and is especially suitable for scaled chicken farms.
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